Lipiidide ja lipoproteiinide biokeemia
Uurimisrühma juht
Seotud struktuuriüksus
TalTech prioriteetne teadussuund
Ülevaade
Uurimisrühma põhitemaatika on seotud lipiidide ja lipoproteiinide metabolismi fundamentaalsete aspektide väljaselgitamisega. Uurimise põhifookuses on lipaaside ja lipoksügenaaside regulatsiooni mehhanismid. Rühmal on kompetents ja kogemused valkude struktuuri ja omaduste uurimises, biomolekulaarsete interaktsioonide analüüsis, ensümoloogias ja lipidiide analüüsis. Uurimistöös kasutatakse massi spektromeetriat, kromatograafiat, kalorimeetriat, pinnaplasmonite resonantsi ning fluorestsentsil põhinevaid tehnoloogiaid.
Uurimisrühma liige
Doktorant
Endised liikmed
Klassifikaator (Frascati)
Võtmesõna
lipiidide ja lipoproteiinide metabolismi regulatsioonimehhanismid
biomolekulaarsed interaktsioonid
lipaasid
lipiidide analüüs
Seotud projektid
Tähtsamad tulemused
2024. a oli uurimisrühma peamine tegevus seotud lipiidide ja lipoproteiinide metabolismi regulatiivsete mehhanismide tuvastamisega ning potentsiaalsete ravimikandidaatide testimisega. Koostöös dr M. Vaheri uurimisrühmaga oleme testinud Eesti taimeekstraktide inhibeerivat toimet pankrease lipaasile kui kaalulanguse sihtvalgule. Kõige tõhusam lipaasi inhibiitor oli kuldjuure ekstrakt. Kasutades oma kalorimeetrilist meetodit, jätkasime lipoproteiini lipaasi aktiivsuse võrdlemist veganite ja kõigesööjate vereplasmas. Olulist erinevust kõigesööjate ja veganite seerumis mõõdetud lipoproteiini lipaasi aktiivsuse keskmises väärtuses ei täheldatud. Järeldame, et lipoproteiini lipaasi aktiivsuse regulatsioonis mängivad olulisemat rolli muud tegurid peale toitumise.
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TalTechi vilistlane Timo Olesk: “Minu juures käivad igal aastal värsked esmaskursuslased ja kunagi ei tea, kas rääkida, kuidas asjad tegelikult olid või olukorda natukene ilustada …” - Bhadoria, R., Ping, K., Lohk, C., Järving, I., Starkov, P. A phenotypic approach to probing cellular outcomes using heterobivalent constructs // Chemical Communications (2020) Vol. 56,30, p. 4216 - 4219.
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