Reproduktiivbioloogia uurimisrühm
TalTech prioriteetne teadussuund
Klassifikaator (Frascati)
Uurimisrühma juht
Uurimisrühma liige
Võtmesõna
Ülevaade
Reproduktiivbioloogia uurimisrühm on keskendunud eelkõige naisepoolse viljatuse molekulaarsete tekkepõhjuste ja diagnostika võimaluste uurimisele. Peamiseks eesmärgiks on kirjeldada rakkudevahelisi molekulaarseid interaktsioone munasarjas ning leida faktorid, mis võimaldaksid eristada arenguvõimelist munarakku hukkumisele määratutest. Uurimistemaatika jaguneb valdavalt kolmeks: (a) inimese munasarja bioloogia ja munasarjapõhise viljatuse uurimine; (b) keskkonnakemikaalide mõju munasarja toimimisele; (c) viljakuse säilitamise meetodite arendamine. Meetoditena kasutatakse kaasaegseid geeniekspressiooni ja rakupopulatsioonide kirjeldamise suundi: süvasekveneerimine, ühe raku analüüsid, funktsionaalsed katsed rakuliinides ja primaarsete rakkude kultuuris jne. Saadud andmeid analüüsitakse ja tulemusi modelleeritakse bioinformaatiliste algoritmide abil.Koostööd tehakse kõikide Eesti viljatusravi kliinikutega, kelle abiga kogutakse bioloogilist materjali (munasarja granuloosa rakud, munasarja biopsiad, follikulaarvedelik, vereproovid) viljakatelt ja viljatutelt naistelt ning Tervisetehnoloogiate Arenduskeskusega.
Seotud struktuuriüksus
Teadusgrupiga seotud publikatsioonid
- Roos, K., Rooda, I., Keif, R.-S., Liivrand, M., Smolander, O.-P., Salumets, A., Velthut-Meikas, A. Single-cell RNA-seq analysis and cell-cluster deconvolution of the human preovulatory follicular fluid cells provide insights into the pathophysiology of ovarian hyporesponse // Frontiers in Endocrinology (2022) vol. 13, art. 945347.
https://doi.org/10.3389/fendo.2022.945347 - Bellavia, A., Zou, R., Bjorvang, R. D., Roos, K., Sjunnesson, Y., Hallberg, I., Vermeulen, R., Salumets, A., Velthut-Meikas, A., Damdimopoulou, P. et al. Association between chemical mixtures and female fertility in women undergoing assisted reproduction in Sweden and Estonia // Environmental research (2023) vol. 216, 1, art. 114447.
https://doi.org/10.1016/j.envres.2022.114447 - Godakumara, K., Ord, J., Lättekivi, F., Dissanayake, K., Viil, J., Boggavarapu, N. R., Faridani, O. R., Jääger, K., Velthut-Meikas, A., Jaakma, Ü., Salumets, A., Fazeli, A. Trophoblast derived extracellular vesicles specifically alter the transcriptome of endometrial cells and may constitute a critical component of embryo-maternal communication // Reproductive biology and endocrinology (2021) vol. 19, 1, art. 115.
https://doi.org/10.1186/s12958-021-00801-5 - Lavogina, D., Stepanjuk, A., Peters, M., Samuel, K., Kasvandik, S., Khatun, M., Arffman, R. K., Enkvist, E., Viht, K., Velthut-Meikas, A. et al. Progesterone triggers Rho kinase-cofilin axis during in vitro and in vivo endometrial decidualization // Human reproduction (2021) vol. 36, 8, p. 2230-2248.
https://doi.org/10.1093/humrep/deab161 - Rooda, I., Kaselt, B., Liivrand, M., Smolander, O.-P., Salumets, A., Velthut-Meikas, A. Hsa-mir-548 family expression in human reproductive tissues // BMC Genomic Data (2021) Vol. 22, Issue 1, Art. 40.
https://doi.org/10.1186/s12863-021-00997-w - Duursen, M.B.M. van, Boberg, J., Christiansen, S., Jääger, K., Salumets, A., Velthut-Meikas, A. et al. Safeguarding female reproductive health against endocrine disrupting chemicals-The FREIA project // International journal of molecular sciences (2020) vol. 21, 9, art. 3215.
https://doi.org/10.3390/ijms21093215 - Kasvandik, S., Saarma, M., Kaart, T., Rooda, I., Velthut-Meikas, A. et al. Uterine fluid proteins for minimally invasive assessment of endometrial receptivity // The journal of clinical endocrinology & metabolism (2020) vol. 105, 1, p. 219-230.
https://doi.org/10.1210/clinem/dgz019 - Johansson, H. K. L., Damdimopoulou, P., Jääger, K., Velthut-Meikas, A. et al. Putative adverse outcome pathways for female reproductive disorders to improve testing and regulation of chemicals // Archives of toxicology (2020) vol. 94, 10, p. 3359–3379.
https://doi.org/10.1007/s00204-020-02834-y - Rooda, I., Hasan, M.M., Roos, K., Viil, J., Smolander, Velthut-Meikas, A. et al. Cellular, extracellular and extracellular vesicular miRNA profiles of pre-ovulatory follicles indicate signaling disturbances in polycystic ovaries // International journal of molecular sciences (2020) vol. 21, 24, art. 9550, 23 p. : ill.
https://doi.org/10.3390/ijms21249550 - Hasan, M.M., Viil, J., Lättekivi, F., Velthut-Meikas, A. et al. Bovine follicular fluid and extracellular vesicles derived from follicular fluid alter the bovine oviductal epithelial cells transcriptome // International journal of molecular sciences (2020) vol. 21, 15, art. 5365 ; 16 p. : ill.
https://doi.org/10.3390/ijms21155365 - Dissanayake, K., Nõmm, M., Lättekivi, F., Velthut-Meikas, A. et al. Oviduct as a sensor of embryo quality: deciphering the extracellular vesicle (EV)-mediated embryo-maternal dialogue // Journal of molecular medicine (2021) vol. 99, issue 5, p. 685–697 : ill.
https://doi.org/10.1007/s00109-021-02042-w - Rooda, I., Hensen, K., Kaselt, B., Velthut-Meikas, A. et al. Target prediction and validation of microRNAs expressed from FSHR and aromatase genes in human ovarian granulosa cells // Scientific reports (2020) vol. 10, 1, art. 2300, 13 p. : ill.
https://doi.org/10.1038/s41598-020-59186-x - Reshi, Q.U.A., Viil, J., Ord, J., Lättekivi, F., Velthut-Meikas, A. et al. Spermatozoa induce transcriptomic alterations in bovine oviductal epithelial cells prior to initial contact // Journal of cell communication and signaling (2020) vol. 14, p. 439–451.
https://doi.org/10.1007/s12079-020-00575-2 - Teder, H., Koel, M., Paluoja, P., Velthut-Meikas, A. et al. TAC-seq : targeted DNA and RNA sequencing for precise biomarker molecule counting // npj genomic medicine (2018) vol. 3, art. 34, 8 p. : ill.
https://doi.org/10.1038/s41525-018-0072-5 - Rekker, K., Altmäe, S., Suhorutshenko, M., Peters, M., Martinez-Blanch, J.F., Codoñer, F.M., Vilella, F., Simón, C., Salumets, A., Velthut-Meikas, A. A two-cohort RNA-seq study reveals changes in endometrial and blood mirnome in fertile and infertile women // Genes (2018) vol. 9, 12, Article nr. 574.
https://doi.org/10.3390/genes9120574 - Suhorutshenko, M., Kukushkina, V., Velthut-Meikas, A., Altmäe, S., Peters, M., Mägi, R., Krjutškov, K., Koel, M., Codoñer, F.M., Martinez-Blanch, J.F. et al. Endometrial receptivity revisited : endometrial transcriptome adjusted for tissue cellular heterogeneity // Human reproduction (2018) vol. 33, 11, 12 p. : ill.
https://doi.org/10.1093/humrep/dey301