Research group of metalloproteomics
TalTech priority area
Research classification (Frascati)
Head of the research group
Research group member
Doctoral students
Keyword
Overview
The Research Group of Metalloproteomics is focused on the following topics: (1) structural andfunctional studies of metalloproteins participating in homeostasis of biometals: zinc, copper andiron by methods like NMR, ESI MS, ICP MS,fluorescence ja UV-VIS spectroscopy; (2) the role of biometals: zinc, copper and iron on fibrillization as well as on in vitro and in vivo toxicity ofamyloigogenic peptides linked with Alzheimer’sdisease; (3) design of new metal-chelating ligandsand elaboration of metal-chelating therapeuticstrategies for Wilsons and Alzheimer’s disease.Professor Palumaa is a member of the EuropeanMolecular Biology Organization (EMBO).
Important results
The most important achievements of the research group during last year were connectedwith the elucidation of the influence of α-lipoicacid on cellular copper metabolism and evaluation of its therapeutic potential in Alzheimer’sdisease insect models.
Kabin, E.; Dong, Y.; Roy, S.; Smirnova, J.; Smith, J. W; Ralle, M.; Summers, K.y; Yang, H.n; Dev, S.; Wang, Y.; Devenney, B.; Cole, R. N; Palumaa, P.; Lutsenko, S. (2023). α-lipoic acid ameliorates consequences of copper overload by up-regulating selenoproteins and decreasing redox misbalance. Proceedings of the National Academy of Sciences, 120 (40), #e2305961120. DOI: 10.1073/pnas.2305961120.
Berntsson, E.; Vosough, F.; Noormägi, A.; Padari, K.; Asplund, F.; Gielnik, M.; Paul, S.; Jarvet, J.; Tõugu, V.; Roos, P. M; Kozak, M.; Gräslund, A.; Barth, A.; Pooga, M.; Palumaa, P.; Wärmländer, S. K T S (2023). Characterization of Uranyl (UO22+) Ion Binding to Amyloid Beta (Aβ) Peptides: Effects on Aβ Structure and Aggregation. ACS Chemical Neuroscience, 14 (15), 2618−2633. DOI: 10.1021/acschemneuro.3c00130.
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Teadusgrupiga seotud publikatsioonid
- Smirnova, J., Gavrilova, J., Noormägi, A., Valmsen, K., Pupart, H., Luo, J., Tõugu, V., Palumaa, P. Evaluation of Zn2+- and Cu2+-binding affinities of native Cu,Zn-SOD1 and its G93A mutant by LC-ICP MS // Molecules (2022) vol. 27, 10, art. 3160.
https://doi.org/10.3390/molecules27103160 - Berntsson, E., Sardis, M., Noormägi, A., Jarvet, J., Roos, P. M., Tõugu, V., Gräslund, A., Palumaa, P., Wärmländer, S. K. T. S. Mercury ion binding to apolipoprotein E variants ApoE2, ApoE3, and ApoE4 : similar binding affinities but different structure induction effects // ACS omega (2022) vol. 7, 33, p. 28924-28931.
https://doi.org/10.1021/acsomega.2c02254 - Friedemann, M., Tõugu, V., Palumaa, P. Cu(II) partially protects three histidine residues and the N-terminus of amyloid-β peptide from diethyl pyrocarbonate (DEPC) modification // FEBS Open Bio (2020) vol. 10, 6, p. 1072-1081.
https://doi.org/10.1002/2211-5463.12857 - Kirsipuu, T., Zadorožnaja, A., Smirnova, J., Friedemann, M.; Plitz, T., Tõugu, V., Palumaa, P. Copper(II)-binding equilibria in human blood // Scientific reports (2020) vol. 10, 1, art. 5686, 10 p. : ill.
https://doi.org/10.1038/s41598-020-62560-4 - Metsla, K., Kirss, S., Laks, K., Sildnik, G., Palgi, M., Palumaa, T., Tõugu, V., Palumaa, P. α-Lipoic acid has the potential to normalize copper metabolism, which is dysregulated in Alzheimer’s disease // Journal of Alzheimer's Disease (2022) vol. 85, 2, p. 715-728.
https://doi.org/10.3233/JAD-215026 - Wallin, C., Friedemann, M., Sholts, S.B., Noormägi, A., Palumaa, P. et al. Mercury and Alzheimer’s disease: Hg(II) ions display specific binding to the amyloid-β peptide and hinder its fibrillization // Biomolecules (2020) vol. 10, 1, art. 44, 23 p. : ill.
https://doi.org/10.3390/biom10010044 - Kubo, A-L., Vasiliev, G., Vija, H., Tõugu, V., Kahru, A. Surface carboxylation or PEGylation decreases CuO nanoparticles’ cytotoxicity to human cells in vitro without compromising their antibacterial properties // Archives of toxicology (2020) vol. 94, p. 1561-1573 : ill.
https://doi.org/10.1007/s00204-020-02720-7 - Koski, L., Ronnevi, C., Berntsson, E., Wärmländer, S.K.T.S., Roos, P.M. Metals in ALS TDP-43 pathology // International Journal of Molecular Sciences (2021) Vo. 22, Issue 22, Art. nr. 12193.
https://doi.org/10.3390/ijms222212193 - Krištal, J., Metsla, K., Bragina, O., Tõugu, V., Palumaa, P. Toxicity of amyloid-β peptides varies depending on differentiation route of SH-SY5Y cells // Journal of Alzheimer's disease (2019) vol. 71, 3, p. 879−887.
https://doi.org/10.3233/JAD-190705 - Smirnova, J., Kabin, E., Järving, I., Bragina, O., Tõugu, V., Plitz, T., Palumaa, P. Copper(I)-binding properties of de-coppering drugs for the treatment of Wilson disease. α-Lipoic acid as a potential anti-copper agent // Scientific reports (2018) 8, 1, art. 1463, 9 p. : ill.
https://doi.org/10.1038/s41598-018-19873-2 - Berntsson, E., Paul, S., Vosough, F., Sholts, S.B., Jarvet, J., Roos, P.M., Barth, A., Gräslund, A., Wärmländer, S.K.T.S. Lithium ions display weak interaction with amyloid-beta (Aβ) peptides and have minor effects on their aggregation // Acta biochimica Polonica (2021) vol. 68, 2, p. 169-179.
https://doi.org/10.18388/abp.2020_5493 - Smirnova, J., Kabin, E., Tõugu, V., Palumaa, P. Redox properties of Cys2His2 and Cys4 zinc fingers determined by electrospray ionization mass spectrometry // FEBS Open Bio (2018) vol. 8, 6, p. 923 - 931.
https.//doi.org/10.1002/2211-5463.12422 - Noormägi, A., Golubeva, T., Berntsson, E., Warmländer, S. K.T.S., Tõugu, V., Palumaa, P. Direct competition of ATCUN peptides with human serum albumin for copper(II) ions determined by LC-ICP MS // ACS omega (2023) vol. 8, 37, p. 33912−33919.
https://doi.org/10.1021/acsomega.3c04649 - Palumaa, P. Metabolism of copper and possibilities for its regulation // Proceedings of the Estonian Academy of Sciences (2023) vol. 72, 4, p. 382-392.
https://doi.org/10.3176/proc.2023.4.03