Systems biology research group

TalTech priority area
Research classification (Frascati)
Head of the research group
intracellular diffusion
fluorescence microscopy
fluorescence correlation spectroscopy
In the Laboratory of Systems Biology, we useinterdisciplinary approaches to tackle questions in cardiac physiology. Our team consistsof researchers with backgrounds in biophysics,biology, and applied mathematics/physics. As aresult, we are able to approach scientific questions on different scales, from organ to molecularlevel, using combinations of different experimental and theoretical techniques by focusing onquantitative analysis of the data.We study diffusion in cardiomyocytes by tracking the movement of fluorescent molecules usingextended raster image correlation spectroscopy.Our results suggest that diffusion barriers are arranged in a 3D lattice with relatively smallopenings. Based on the analysis of autofluorescence response, we demonstrated that mitochondrial outer membrane and cytosolic diffusionbarriers reduce the movement of molecules toa similar extent. We study effects of creatinedeficiency to establish the role of creatine kinaseshuttle in the heart. In the research papers published this year, we demonstrated how creatinedeficiency leads to intracellular adaptations interms of alternative energy transfer systemschanges and an impact on calcium handlingof cardiomyocytes. These studies form a basisfor our current line of research on interactionsbetween processes in cardiomyocytes.
Important results
We have been active in the development of newtechniques and distribute them as open-sourcetools: deconvolution software for enhancingconfocal imaging, symbolic flux analysis forgenome-scale metabolic networks, and real-timesarcomere length estimation techniques. This development work has raised interest in companieswith an outreach of incorporating our algorithmsand software into their products.
Period of activity of the research group
Related department
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