Mikrofluidika uurimisrühm
TalTech prioriteetne teadussuund
TalTechDigital
Klassifikaator (Frascati)
Uurimisrühma juht
Uurimisrühma liige
Doktorant
Võtmesõna
tilga mikrofluidika
antibiootikumi resistentsus
antibiotic resistance
mikrobioloogia
pildianalüüs
Ülevaade
Mikrofluidika aitab teha keemilisi ja bioloogilisi katseid hästi väikestes vedelike mahtudes. Tavaliselt on nii rakendus- kui alusuuringutes tehtavate katsete mahud laboris vahemikus ühest liitrist mikroliitrini. Mikrofluidika seadmed võimaldavad automatiseerida, kontrollida ja teha katseid veelgi väiksemates mahtudes. Mikrofluidika seadmete konstrueerimine on multidistsiplinaarne valdkond, kuhu panustavad nii mehhaanika, IT, inseneeria, materjaliteadus, keemia, bioloogia ja paljud teised valdkonnad.
Tähtsamad tulemused
Uurimisrühm on välja töötanud kasutajasõbralikud ja kiired töövahendid tilga mikrofluidikas. Vesi-õlis tilgad on nagu väikesed katseklaasid, mida kasutatakse keemias ja biotehnoloogias. Sellistes eksperimentides võib olla korraga miljoneid tilku ning nende kiireks analüüsiks ongi loodud erinevaid kasutajasõbralikke open-source tööriistu. 2023. aastal töötasid uurimisrühma teadlased välja kasutajasõbralikud tööriistad nanoliitriste katseklaaside analüüsimiseks, mis peagi võidakse igapäevaselt kasutusele võtta nii teaduslaborites kui igal pool mujal. Nanoliitriste katseklaasidena kasutatakse vesi-õlis tilkade emulsiooni, kus veetilk on nagu väikene katseklaas.
Gyimah, N.; Scheler, O.; Rang, T.; Pardy, T. (2023). Deep reinforcement learning-based digital twin for droplet microfluidics control. Physics of Fluids, 35 (8), ARTN 082020. DOI: 10.1063/5.0159981.
Seotud projektid
Uute mikrofluidika tehnoloogiate arendamine keemias ja bioloogias (TTÜ arenguprogramm aastateks 2016-2022)
Seotud struktuuriüksus
- Sanka, I., Bartkova, S., Pata, P., Smolander, O.P., Scheler, O. Investigation of different free image analysis software for high-throughput droplet detection // ACS omega (2021) Vol. 6, 35, p. 22625-22634 : ill.
https://doi.org/10.1021/acsomega.1c02664 - Bartkova, S., Vendelin, M., Sanka, I., Pata, P., Scheler, O. Droplet image analysis with user-friendly freeware CellProfiler // Analytical methods (2020) vol. 12, 17, p. 2287-2294 : ill.
https://doi.org/10.1039/D0AY00031K - Scheler, O., Makuch, K., Debski, P. R., Smolander, O.-P. et al. Droplet-based digital antibiotic susceptibility screen reveals singlecell clonal heteroresistance in an isogenic bacterial population // Scientific reports (2020) vol. 10, 1, art. 3282, 8 p. : ill.
https://doi.org/10.1038/s41598-020-60381-z - Lund, P. A., De Biase, D., Liran, O., Scheler, O. et al. Understanding How Microorganisms Respond to Acid pH Is Central to Their Control and Successful Exploitation // Frontiers in microbiology (2020) vol. 11, art. 556140, 8 p. : ill.
https://doi.org/10.3389/fmicb.2020.556140 - Bartkova, S., Kahru, A., Heinlaan, M., Scheler, O Techniques used for analyzing microplastics, antimicrobial resistance and microbial community composition : a mini-review // Frontiers in microbiology (2021) vol. 12, art. 603967.
https://doi.org/10.3389/fmicb.2021.603967 - Gyimah, N., Scheler, O., Rang, T., Pardy, T. Can 3D printing bring droplet microfluidics to every lab? - A systematic review // Micromachines (2021) vol. 12, 3, art. 339.
https://doi.org/10.3390/mi12030339 - Kaljurand, M., Mazina-Šinkar, J. Portable capillary electrophoresis as a green analytical technology // TrAC Trends in Analytical Chemistry (2022) vol. 157, art. 116811.
https://doi.org/10.1016/j.trac.2022.116811 - Lazouskaya, M., Scheler, O., Mikli, V., Uppuluri, K., Zaraska, K., Tamm, M. Nafion protective membrane enables using ruthenium oxide electrodes for pH measurement in milk // Journal of The Electrochemical Society (2021) vol. 168, 10, art. 107511, 12 p. : ill.
https://doi.org/10.1149/1945-7111/ac2d3c - Kaljurand, M., Saar-Reismaa, P., Vaher, M., Gorbatšova, J., Mazina-Šinkar, J. Capillary electrophoresis as a monitoring tool for flow composition determination // Molecules (2021) vol. 26, 16, art. 4918, 12 p. : ill.
https://doi.org/10.3390/molecules26164918 - Kaljurand, M., Gorbatšova, J., Mazina-Šinkar, J. A gas chromatograph for citizen science // Microchemical journal (2021) vol. 165, art. 106195, 6 p. : ill.
https://doi.org/10.1016/j.microc.2021.106195 - Pärnamets, K., Pardy, T., Koel, A., Rang, T., Scheler, O., Le Moullec, Y., Afrin, F. Optical detection methods for high-throughput fluorescent droplet microflow cytometry // Micromachines (2021) vol. 12, 3, art. 345, 20 p. : ill.
https://doi.org/10.3390/mi12030345 - Lazouskaya, M., Tamm, M., Scheler, O. et al. Nafion as a protective membrane for screen-printed pH-sensitive ruthenium oxide electrodes // 2020 17th Biennial Baltic electronics conference, Tallinn, Estonia, October 6-8, 2020 : proceedings. Danvers : IEEE, 2020. 4 p. : ill.
https://doi.org/10.1109/BEC49624.2020.9276822 - Gyimah, N., Scheler, O., Rang, T., Pardy, T. Digital twin for controlled generation of water-in-oil microdroplets with required size // 2022 23rd International Conference on Thermal, Mechanical and Multi-Physics Simulation and Experiments in Microelectronics and Microsystems (EuroSimE), 25-27 April 2022, St Julian, Malta : proceedings. : IEEE, 2022. p. 85-91.
https://doi.org/10.1109/EuroSimE54907.2022.9758876 - Ruszczak, A., Bartkova, S., Zapotoczna, M., Scheler, O., Garstecki, P. Droplet-based methods for tackling antimicrobial resistance // Current opinion in biotechnology (2022) vol. 76, art. 102755.
https://doi.org/10.1016/j.copbio.2022.102755 - Ružicka, M., Kaljurand, M., Gorbatšova, J., Mazina-Šinkar, J. Autosampler for portable capillary electrophoresis // Journal of chromatography A (2022) vol. 1685, art. 463619.
https://doi.org/10.1016/j.chroma.2022.463619 - Shalima, T., Mishra, K.A., Kaabel, S., Ustrnul, L., Bartkova, S., Tõnsuaadu, K., Heinmaa, I., Aav, R. Cyclohexanohemicucurbit[8]uril inclusion complexes with heterocycles and selective extraction of sulfur compounds from water // Frontiers in chemistry (2021) Vol. 9, art. 786746, 8 p. : ill.
https://doi.org/10.3389/fchem.2021.786746 - Lazouskaya, M., Stulova, I., Sõrmus, A., Scheler, O., Tiisma, K., Vinter, T., Loov, R., Tamm, M. Front-face fluorimeter for the determination of cutting time of cheese curd // Foods (2021) vol. 10, 3, art. 576, 13 p.
https://doi.org/10.3390/foods10030576 - Teras, J., Teras, M., Pata P., Mägi A., et al. First Eastern European experience of isolated limb infusion for in-transit metastatic melanoma confined to the limb: Is it still an effective treatment option in the modern era? // European journal of surgical oncology (2020) vol. 46, 2, p. 272-276.
https://doi.org/10.1016/j.ejso.2019.10.039 - Gyimah, N., Jõemaa, R., Pärnamets, K., Scheler, O., Rang, T., Pardy, T. PID controller tuning optimization using genetic algorithm for droplet size control in microfluidics // 2022 18th Biennial Baltic Electronics Conference (BEC). : IEEE, 2022. 6 p.
https://doi.org/10.1109/BEC56180.2022.9935596 - Ružicka, M., Kaljurand, M., Gorbatšova, J., Vaher, M., Mazina-Šinkar, J. Portable fully automated oral fluid extraction device for illegal drugs // Talanta (2022) vol. 243, art. 123389.
https://doi.org/10.1016/j.talanta.2022.123389 - Bimbiraite-Surviliene, K., Drevinskas, T., Maruska, A., Gorbatsova, J., Kaljurand, M. et al. Portable automated handheld sample collection-preparation instrument for airborne volatile substances // Microchemical journal (2020) vol. 159, art. 105576.
https://doi.org/10.1016/j.microc.2020.105576 - Bolkvadze, V., Bondar, D., Vaher, M., Halling, E., Gorbatsova, J., Mazina-Šinkar, J. The influence of organic solvents on phenylethylamines in capillary zone electrophoresis // Journal of chromatography A (2022) vol. 1675, art. 463169, 9 p. : ill.
https://doi.org/10.1016/j.chroma.2022.463169 - Azizi, T., Carvalho De Araujo, L., Cetecioglu, Z., Clancy, A. J., Feger, M. L., Liran, O., O’Byrne, C., Sanka, I., Scheler, O., Sedlakova-Kadukova, J., Ziv, C., De Biase, D., Lund, P. A. A COST Action on microbial responses to low pH : developing links and sharing resources across the academic-industrial divide // New biotechnology (2022) vol. 72, p. 64-70.
https://doi.org/10.1016/j.nbt.2022.09.002 - Lazouskaya, M., Scheler, O., Uppuluri, K., Zaraska, K., Tamm, M. Reusability of RuO2-Nafion electrodes, suitable for potentiometric pH measurement // 2022 IEEE International Conference on Flexible and Printable Sensors and Systems (FLEPS), Vienna, Austria, 2022. : IEEE, 2022. p. 1-4.
https://doi.org/10.1109/FLEPS53764.2022.9781521 - Sanka, I., Bartkova, S., Pata, P, Smolander, O.-P., Scheler, O. High-throughput pipeline for polydisperse droplet analysis // MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. : Chemical and Biological Microsystems Society, 2021. p. 563-564.
https://microtas2021.org/program/MicroTAS2021_Program.pdf - Lazouskaya, M., Vetik, I., Uppuluri, K., Razmi, N., Scheler, O. Cleaning procedure for the screen-printed RuO2 pH electrodes // IEEE Sensors 2022 : Dallas, Texas, USA : 30 October 2022 - 02 November 2022 : Sensors 2022 conference proceedings. : IEEE, 2022. 4 p. : ill.
https://doi.org/10.1109/SENSORS52175.2022.9967177 - Bartkova, S., Vendelin, M., Pata, P., Scheler, O. Cellprofiler is a fit tool for droplet digital image analysis // 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019) Basel, Switzerland, 27 – 31 October 2019. New York : Chemical and Biological Microsystems Society, 2020. art. 163704, p. 1644-1645.
https://doi.org/10.1101/811869 - Reinsalu, O., Scheler, O., Mikelsaar, R., Mikelsaar, A.-V., Hallap, T., Jaakma, Ü., Padrik, P., Kavak, A., Salumets, A., Kurg, A. A dual colour FISH method for routine validation of sexed Bos taurus semen // BMC Veterinary Research (2019) Vol. 15, 1, art. 104.
https://doi.org/10.1186/s12917-019-1839-3 - Teras, J., Magi, A., Teras, M., Pata, P. et al. Soft tissue cancer management: isolated limb infusion for sarcoma // Visceral medicine (2019) vol. 35, 6, p. 373-379 : ill.
https://doi.org/10.1159/000495888 - Teras, M., Viisileht, E., Pahtma-Hall, M., Rump, A., Paalme, V., Pata, P., Pata, I., Langevin, C., Boudinot Ruutel, S. Porcine circovirus type 2 ORF3 protein induces apoptosis in melanoma cells // BMC cancer (2018) Vol. 18, Issue 1, art. 1237, 12 p. : ill.
https://doi.org/10.1186/s12885-018-5090-2 - Pacocha, N., Scheler, O., Nowak, M. M. et al. Direct droplet digital PCR (dddPCR) for species specific, accurate and precise quantification of bacteria in mixed samples // Analytical methods (2019) vol. 11, 44, p. 5655–5738 : ill.
https://doi.org/10.1039/c9ay01874c - Drevinskas, T., Telksnys, L., Maruška, A., Gorbatšova, J., Kaljurand, M. Compensation of the baseline temperature fluctuations for autonomous CE–C4D instrument working in harsh environments // Electrophoresis (2018) vol. 39, 22, p. 2877–2883 : ill.
https://doi.org/10.1002/elps.201800132 - Drevinskas, T., Telksnys, L., Maruška, A., Gorbatšova, J., Kaljurand, M. Capillary electrophoresis sensitivity enhancement based on adaptive moving average method // Analytical chemistry (2018) vol. 90, 11, p. 6773−6780 : ill.
https://doi.org/10.1021/acs.analchem.8b00664 - Postek, W., Gargulinski, P., Scheler, O., Kaminski, T., Garstecki, P. Microfluidic screening of antibiotic susceptibility at a single-cell level shows the inoculum effect of cefotaxime on: E. coli // Lab on a Chip (2018) Vol. 18, Issue 23, p. 3668 - 3677.
https://doi.org/10.1039/c8lc00916c - Kovacovicova, K.; Školnaja, M.; Heinmaa, M.; Mistrik, M.; Pata, P.; Pata, I. et al. Senolytic cocktail Dasatinib+Quercetin (D+Q) does not enhance the efficacy of senescence-inducing chemotherapy in liver cancer [Online resource] // Frontiers in oncology (2018) vol. 8, art. 459, 7 p. : ill.
https://doi.org/10.3389/fonc.2018.00459 - Drevinskas, T., Maruška, A., Girdauskas, V., Dūda, G., Gorbatsova, J., Kaljurand, M. Complete capillary electrophoresis process on a drone : towards a flying micro-lab // Analytical Methods (2020) Vol. 12, 41, p. 4977 - 4986.
https://doi.org/10.1039/d0ay01220c - Giallongo, S., Řeháková, D., Biagini, T., Lo Re, O., Raina, P., Lochmanová, G., Zdráhal, Z., Resnick, I., Pata, P., Pata, I., Mistrík, M., De Magalhães, J., Mazza, T., Koutná, I., Vinciguerra, M. Histone variant macroH2A1.1 Enhances nonhomologous end joining-dependent DNA double-strand-break repair and reprogramming efficiency of human iPSCs // Stem Cells (2022) vol. 40, 1, p. 35 - 48.
https://doi.org/10.1093/stmcls/sxab004 - Kaljurand, M., Ružička, M., Gorbatšova, J., Mazina-Šinkar, J. New developments in separation science will help to contribute to the democratisation of analytical chemistry // Microchemical journal (2023) vol. 195, Art. 109443.
https://doi.org/10.1016/j.microc.2023.109443 - Kaljurand, M., Růžička, M., Gorbatsova, J., Mazina-Šinkar, J. Evaluation of different operating modes of an autosampler for portable capillary electrophoresis // Journal of Chromatography A (2023) vol. 1705, art. 464201.
https://doi.org/10.1016/j.chroma.2023.464201 - Drevinskas, T., Maruška, A., Gladkauskas, E., Telksnys, L., Girdauskas, V., Gorbatsova, J., Kaljurand, M., Ragažinskienė, O. Design and applications of miniaturized, portable LED based colorimeter // Chemija (2018) vol. 29, 4, p. 209 - 218.
https://doi.org/10.6001/chemija.v29i4.3836 - Ruszczak, A., Jankowski, P., Vasantham, S. K., Scheler, O., Garstecki, P. Physicochemical Properties Predict Retention of Antibiotics in Water-in-Oil Droplets // Analytical chemistry (2023) vol. 95, 2, p. 1574−1581 : ill.
https://doi.org/10.1021/acs.analchem.2c04644 - Kasprzak, M. M., Sady, M., Kruk, J., Bartkova, S., Sanka, I., Scheler, O., Jamroz, E., Berski, W., Onacik-Guer, S. et al. Replacement of milk fat by rapeseed oil stabilised emulsion in commercial yogurt // PeerJ (2023) vol. 11, art. e16441.
https://doi.org/10.7717/peerj.16441 - Drevinskas, T., Maruška, A., Naujokaitytė, G., Telksnys, L., Kaljurand, M., Stanys, V., Cowles, J., Gorbatsova, J. Towards adaptive method for peak migration time correction : discretization period in electropherograms // Chemija (2020) vol. 31, 3, p. 146-155 : ill.
https://doi.org/10.6001/CHEMIJA.V31I3.4288 - Lazouskaya, M., Vetik, I., Tamm, M., Uppuluri, K., Scheler, O. Binary RuO2–CuO electrodes outperform RuO2 electrodes in measuring the pH in food samples // ACS omega (2023) vol. 8, 14, p. 13275-13284.
https://doi.org/10.1021/acsomega.3c00538 - Jõemaa, R., Gyimah, N., Ashraf, K., Pärnamets, K., Zaft, A., Scheler, O., Rang, T., Pardy, T. Cogniflow-drop : integrated modular system for automated generation of droplets in microfluidic applications // IEEE Access (2023) vol. 11, p. 104905-104929.
https://doi.org/10.1109/ACCESS.2023.3316726 - Sanka, I., Bartkova, S., Pata, P., Ernits, M., Meinberg, Monika M., Agu, N., Aruoja, V., Smolander, O-P., Scheler, O. User-friendly analysis of droplet array images // Analytica chimica acta (2023) vol. 1272, art. 341397.
https://doi.org/10.1016/j.aca.2023.341397