Instrumental analysis
TalTech priority area
Research classification (Frascati)
Head of the research group
Research group member
Doctoral students
Keyword
separation methods
miniaturization
phytochemicals
antioxidativity
biomass
ionic liquids
deep eutectic solvents
banned chemicals
Overview
The members of the group have competence indevelopment of analytical methods and procedures and use of these in analysis of differentcompounds and mixtures in complicated matrices. The group has good specialists in separationmethods: gas and liquid chromatography andespecially in capillary electrophoresis where theyuse a wide variety of detectors: electrical, opticaland mass spectrometrical. The group has alsocompetence and means for supercritical extraction for a wide range of extraction parameters.The group has provided recognised results ondevelopment of porous materials – aerogels andthese have been taken into use as adsorbents inanalytical separation and catalysts in electrochemistry and water purification.The aim of the present research is developmentand application of a variety of analytical methodologies (primarily capillary electrophoresis)for analysis of different classes of compounds(banned chemicals, drugs, polyphenols, fermentable sugars) in complex matrixes such asenvironmental samples, body fluids, biomass andherbal extracts. The results obtained will be usedto develop an expert system using quantitativestructure-activity relationship (QSAR).Miniaturization of capillary electrophoresis apparatus is an important feature, which providesan opportunity to perform on-site analyzes.
Important results
Most important part of the research was connected to the development and evaluation of an automatic portable capillary electrophoresis instrument, prototype II of Narcotester (DrugHunter). The CE system was coupled to UVC fluorescence detector providing 225–255 nm exitation wavelength range for the determination of the abuse of illegal drugs in oral fluid in situ. This portable Narcotester was employed for determination of natively fluorescensing drugs,such as cocaine, cocaethylene, seven amphetamines, fentanyl, LSD, tetrahydrocannabinol and cannabidiol. Based on this portable apparatus,several electrophoretic methodologies were developed, validated and successfully utilized for the determination of illegal abuse of drugs during music festivals in Estonia and Latvia.
Related projects
Related department
Publications related to the research group
- Saar-Reismaa, P., Bragina, O., Kuhtinskaja, M., Reile, I., Laanet, P.-R., Kulp, M., Vaher, M. Extraction and fractionation of bioactives from Dipsacus fullonum L. leaves and evaluation of their anti-borrelia activity // Pharmaceuticals (2022) vol. 15, 1, 12 p. : ill.
https://doi.org/10.3390/ph15010087 - Laanet, P.-R., Vaher, M., Saar-Reismaa, P. Micellar electrokinetic chromatography method for the analysis of synthetic and phytocannabinoids // Journal of chromatography A (2022) vol. 1673, art. 463080.
https://doi.org/10.1016/j.chroma.2022.463080 - Saar-Reismaa, P., Koel, M., Tarto, R., Vaher, M. Extraction of bioactive compounds from Dipsacus fullonum leaves using deep eutectic solvents // Journal of chromatography A (2022) vol. 1677, art. 463330.
https://doi.org/10.1016/j.chroma.2022.463330 - Kaljurand, M., Saar-Reismaa, P., Vaher, M., Gorbatšova, J., Mazina-Šinkar, J. Capillary electrophoresis as a monitoring tool for flow composition determination // Molecules (2021) vol. 26, 16, art. 4918, 12 p. : ill.
https://doi.org/10.3390/molecules26164918 - Kaljurand, M., Gorbatšova, J., Mazina-Šinkar, J. A gas chromatograph for citizen science // Microchemical journal (2021) vol. 165, art. 106195, 6 p. : ill.
https://doi.org/10.1016/j.microc.2021.106195 - Jõul, P., Vaher, M., Kuhtinskaja, M. Carbon aerogel-based solid-phase microextraction coating for the analysis of organophosphorus pesticides // Analytical methods (2021) vol. 13, 1, p. 69−76 : ill.
https://doi.org/10.1039/D0AY02002H - Saar-Reismaa, P., Brilla, C.A., Leiman, K., Kaljurand, M., Vaher, M., Kulp, M., Mazina-Šinkar, J. Use of a newly-developed portable capillary electrophoresis analyser to detect drugs of abuse in oral fluid: A case study // Talanta (2020) vol. 211, art. 120662, 9 p.
https://doi.org/10.1016/j.talanta.2019.120662 - Koel, M., Kuhtinskaja, M., Vaher, M. Extraction of bioactive compounds from Catharanthus roseus and Vinca minor // Separation and purification technology (2020) vol. 252, 1, art. 117438 ; 5 p. : ill.
https://doi.org/10.1016/j.seppur.2020.117438 - Usmani, Z., Kulp, M., Lukk, T. Bioremediation of lindane contaminated soil: Exploring the potential of Actinobacterial strains // Chemosphere (2021) vol. 278, art. 130468, 12 p. : ill.
https://doi.org/10.1016/j.chemosphere.2021.130468 - Saar-Reismaa, P., Kotkas, K., Rosenberg, V., Kulp, M., Kuhtinskaja, M., Vaher, M. Analysis of total phenols, sugars, and mineral elements in colored tubers of solanum tuberosum l. // Foods (2020) vol. 9, 12, art. 1862.
https://doi.org/10.3390/foods9121862 - Kaljurand, M., Mazina-Šinkar, J. Portable capillary electrophoresis as a green analytical technology // TrAC Trends in Analytical Chemistry (2022) vol. 157, art. 116811.
https://doi.org/10.1016/j.trac.2022.116811 - Jõul, P., Ho, T. T., Kallavus, U., Konist, A., Leiman, K., Salm, O-S., Kulp, M., Koel, M., Lukk, T. Characterization of organosolv lignins and their application in the preparation of aerogels // Materials (2022) vol. 15, 8, art. 2861.
https://doi.org/10.3390/ma15082861 - Koel, M., Kaljurand, M. Editorial overview : a closer look on green developments in analytical chemistry: green analytical chemistry is going mainstream // Current Opinion in Green and Sustainable Chemistry (2021) vol. 31, Art. 100541.
https://doi.org/10.1016/j.cogsc.2021.100541 - Kuhtinskaja, M., Bragina, O., Kulp, M., Vaher, M. Anticancer Effect of the Iridoid Glycoside Fraction from Dipsacus fullonum L. Leaves // Natural product communications (2020) vol. 15, 9, p. 1-6.
https://doi.org/10.1177/1934578X20951417 - Jurjeva, J., Koel, M. Implementing greening into design in analytical chemistry // Talanta open (2022) vol. 6, art. 100136, 7 p.
https://doi.org/10.1016/j.talo.2022.100136 - Erkhova, L.V., Presniakov, I.A., Afanasov, M.I., Danilson, M., Koel, M. et al. Ferrocene introduced into 5-methylresorcinol-based organic aerogels // Polymers (2020) vol. 12, 7, art. 1582 ; 12 p. : ill.
https://doi.org/10.3390/polym12071582 - Ružicka, M., Kaljurand, M., Gorbatšova, J., Mazina-Šinkar, J. Autosampler for portable capillary electrophoresis // Journal of chromatography A (2022) vol. 1685, art. 463619.
https://doi.org/10.1016/j.chroma.2022.463619 - Ružicka, M., Kaljurand, M., Gorbatšova, J., Vaher, M., Mazina-Šinkar, J. Portable fully automated oral fluid extraction device for illegal drugs // Talanta (2022) vol. 243, art. 123389.
https://doi.org/10.1016/j.talanta.2022.123389 - Lees, H., Jõul, P., Siilak, K., Vaher, M. Separation of perfluoroalkyl substances by using nonaqueous capillary electrophoresis with conductivity detection // Separation science plus (2020) vol. 3, 7, p. 313-320.
https://doi.org/10.1002/sscp.202000016 - Prits, A.-V., Nerut, J., Kasuk, H., Koel, M., Sepp, S., Valk, P., Aruväli, J., Koppel, M., Mikli, V., Volobujeva, O., Lust, E. Carbon aerogel platinum-praseodymium oxide nanocatalyst for methanol oxidation in 0.5 M sulfuric acid : (digital presentation) // ECS transactions (2022) vol. 108, 7, art. 79.
https://doi.org/10.1149/10807.0079ecst - Bolkvadze, V., Bondar, D., Vaher, M., Halling, E., Gorbatsova, J., Mazina-Šinkar, J. The influence of organic solvents on phenylethylamines in capillary zone electrophoresis // Journal of chromatography A (2022) vol. 1675, art. 463169, 9 p. : ill.
https://doi.org/10.1016/j.chroma.2022.463169 - Jurjeva, J., Koel, M. The chemometric approach to identification of residual oil contamination at former primitive asphalt pavement plants // Oil shale (2019) vol. 36, 3, p. 410-430 : ill.
http://www.kirj.ee/32501/?tpl=1061&c_tpl=1064 https://doi.org/10.3176/oil.2019.3.04 - Lees, H., Zapata, F., Vaher, M., García-Ruiz, C. Study of the adhesion of explosive residues to the finger and transfer to clothing and luggage // Science & Justice (2018) vol. 58, 6, p. 415-424.
https://doi.org/10.1016/j.scijus.2018.07.002 - Lees, H., Zapata, F., Vaher, M., García-Ruiz, C. Simple multispectral imaging approach for determining the transfer of explosive residues in consecutive fingerprints // Talanta (2018) vol. 184, p. 437-445 : ill.
https://doi.org/10.1016/j.talanta.2018.02.079 - Söderström, M., Östin, A., Vaher, M., Jõul, P., Lees, H., Kaljurand, M. et al. Chemical analysis of dumped chemical warfare agents during the MODUM project // Towards the monitoring of dumped munitions threat (MODUM) : a study of chemical munitions dumpsites in the Baltic Sea. Dordrecht : Springer, 2018. p. 71-103. (NATO science for peace and security series. C, Environmental security).
https://doi.org/10.1007/978-94-024-1153-9_4 - Krištal, J., Metsla, K., Bragina, O., Tõugu, V., Palumaa, P. Toxicity of amyloid-β peptides varies depending on differentiation route of SH-SY5Y cells // Journal of Alzheimer's disease (2019) vol. 71, 3, p. 879−887.
https://doi.org/10.3233/JAD-190705 - Aid, T., Koel, M., Lopp, M., Vaher, M. Metal-catalyzed degradation of cellulose in ionic liquid media // Inorganics (2018) vol. 6, 3, art. 78, 11 p. : ill.
https://doi.org/10.3390/inorganics6030078 - Jõul, P., Vaher, M., Kuhtinskaja, M. Evaluation of carbon aerogel-based solid-phase extraction sorbent for the analysis of sulfur mustard degradation products in environmental water samples // Chemosphere (2018) Vol. 198, p. 460-468.
https://doi.org/10.1016/j.chemosphere.2018.01.157 - Kaljurand, M. Paper microzones as a route to greener analytical chemistry // Current Opinion in Green and Sustainable Chemistry (2019) Vol. 19, p. 15-18.
https://doi.org/10.1016/j.cogsc.2019.03.002 - Drevinskas, T., Telksnys, L., Maruška, A., Gorbatšova, J., Kaljurand, M. Compensation of the baseline temperature fluctuations for autonomous CE–C4D instrument working in harsh environments // Electrophoresis (2018) vol. 39, 22, p. 2877–2883 : ill.
https://doi.org/10.1002/elps.201800132 - Bolobajev, J., Kask, M., Kreek, K., Kulp, M., Koel, M., Goi, A. Metal-doped organic aerogels for photocatalytic degradation of trimethoprim // Chemical engineering journal (2019) vol. 357, p. 120-128 : ill.
https://doi.org/10.1016/j.cej.2018.09.127 Tehnikaülikooli teadlaste meetod aitab puhastada reovett antibiootikumijääkidest https://keskkonnatehnika.ee/reovee-puhastamine-kasutades-aerogeele/ - Drevinskas, T., Telksnys, L., Maruška, A., Gorbatšova, J., Kaljurand, M. Capillary electrophoresis sensitivity enhancement based on adaptive moving average method // Analytical chemistry (2018) vol. 90, 11, p. 6773−6780 : ill.
https://doi.org/10.1021/acs.analchem.8b00664 - Saar-Reismaa, P., Tretjakova, A., Mazina-Šinkar, J., Vaher, M., Kaljurand, M., Kulp, M. Rapid and sensitive capillary electrophoresis method for the analysis of Ecstasy in an oral fluid // Talanta (2019) vol. 197, p. 390-396 : ill.
https://doi.org/10.1016/j.talanta.2019.01.029 - Saar-Reismaa, P., Erme, E., Vaher, M., Kulp, M., Kaljurand, M., Mazina-Šinkar, J. In situ determination of illegal drugs in oral fluid by portable capillary electrophoresis with deep UV excited fluorescence detection // Analytical chemistry (2018) vol. 90, 10, p. 6253-6258 : ill.
https://doi.org/10.1021/acs.analchem.8b00911 - Smirnova, J., Kabin, E., Järving, I., Bragina, O., Tõugu, V., Plitz, T., Palumaa, P. Copper(I)-binding properties of de-coppering drugs for the treatment of Wilson disease. α-Lipoic acid as a potential anti-copper agent // Scientific reports (2018) 8, 1, art. 1463, 9 p. : ill.
https://doi.org/10.1038/s41598-018-19873-2 - Drevinskas, T., Maruška, A., Girdauskas, V., Dūda, G., Gorbatsova, J., Kaljurand, M. Complete capillary electrophoresis process on a drone : towards a flying micro-lab // Analytical Methods (2020) Vol. 12, 41, p. 4977 - 4986.
https://doi.org/10.1039/d0ay01220c - Bonetta, R., Hunter, G.J., Trinh, C.H., Borowski, T., Fenech, A.G., Kulp, M., Tabares, L.C., Un, S., Hunter, T. Substitution of histidine 30 by asparagine in manganese superoxide dismutase alters biophysical properties and supports proliferation in a K562 leukemia cell line // European biophysics journal (2021) vol. 50, 3-4, p. 571-585 : ill.
https://doi.org/10.1007/s00249-021-01544-2 - Laanet, Pille-Riin, Saar-Reismaa, Piret, Jõul, Piia, Bragina, Olga, Vaher, Merike Phytochemical screening and antioxidant activity of selected Estonian Galium species // Molecules (2023) vol. 28, 6, art. 2867.
https://doi.org/10.3390/molecules28062867 - Bljahhina, A., Pismennõi, D., Kriščiunaite, T., Kuhtinskaja, M., Kobrin, E.-G. Quantitative analysis of oat (Avena sativa L.) and pea (Pisum sativum L.) saponins in plant-based food products by hydrophilic interaction liquid chromatography coupled with mass spectrometry // Foods (2023) vol. 12, 5, art. 991, 16 p. : ill.
https://doi.org/10.3390/foods12050991 - Kaljurand, M., Růžička, M., Gorbatsova, J., Mazina-Šinkar, J. Evaluation of different operating modes of an autosampler for portable capillary electrophoresis // Journal of Chromatography A (2023) vol. 1705, art. 464201.
https://doi.org/10.1016/j.chroma.2023.464201 - Drevinskas, T., Maruška, A., Gladkauskas, E., Telksnys, L., Girdauskas, V., Gorbatsova, J., Kaljurand, M., Ragažinskienė, O. Design and applications of miniaturized, portable LED based colorimeter // Chemija (2018) vol. 29, 4, p. 209 - 218.
https://doi.org/10.6001/chemija.v29i4.3836 - Lees, H., Jõul, P., Pikkor, H., Järvik, O., Mets, B., Konist, A. Characterization of oil shale kerogen semi-coke and its application to remove chemical pollutants from aqueous solutions // Oil shale (2023) vol. 40, 2, p. 115-132 : ill.
https://doi.org/10.3176/oil.2023.2.02 - Kaljurand, M., Ružička, M., Gorbatšova, J., Mazina-Šinkar, J. New developments in separation science will help to contribute to the democratisation of analytical chemistry // Microchemical journal (2023) vol. 195, Art. 109443.
https://doi.org/10.1016/j.microc.2023.109443 - Bimbiraite-Surviliene, K., Drevinskas, T., Maruska, A., Gorbatsova, J., Kaljurand, M. et al. Portable automated handheld sample collection-preparation instrument for airborne volatile substances // Microchemical journal (2020) vol. 159, art. 105576.
https://doi.org/10.1016/j.microc.2020.105576 - Bljahhina, A., Kuhtinskaja, M., Kriščiunaite, T. Development of extraction method for determination of saponins in soybean-based yoghurt alternatives: effect of sample pH // Foods (2023) vol. 12, 11, art. 2164.
https://doi.org/10.3390/foods12112164 - Drevinskas, T., Maruška, A., Naujokaitytė, G., Telksnys, L., Kaljurand, M., Stanys, V., Cowles, J., Gorbatsova, J. Towards adaptive method for peak migration time correction : discretization period in electropherograms // Chemija (2020) vol. 31, 3, p. 146-155 : ill.
https://doi.org/10.6001/CHEMIJA.V31I3.4288 - Tavares, W. R., Jiménez, I. A., Oliveir, L., Kuhtinskaja, M., Vaher, M., Ros, J. S., Seca, A. M. L., Bazzocchi, I. L., Barreto, M. C. Macaronesian plants as promising biopesticides against the crop pest Ceratitis capitata // Plants (2023) vol. 12, 24, art. 4122.
https://doi.org/10.3390/plants12244122 - Berikashvili, V., Khardziani, T., Kobakhidze, A., Kulp, M., Kuhtinskaja, M., Lukk, T., Gargano, M. L., Venturella, G., Kachlishvili, E., Metreveli, E. et al. Antifungal activity of medicinal mushrooms and optimization of submerged culture conditions for Schizophyllum commune (Agaricomycetes) // International journal of medicinal mushrooms (2023) vol. 25, 10, p. 1-21.
https://doi.org/10.1615/IntJMedMushrooms.2023049836 - Jõul, P., Järvik, O., Lees, H., Kallavus, U., Koel, M., Lukk, T. Preparation and characterization of lignin-derived carbon aerogels // Frontiers in chemistry (2023) vol. 11, art. 1326454.
https://doi.org/10.3389/fchem.2023.1326454