Instrumentaalanalüüs

Nimetus
Instrumental analysis
Ülevaade
Uurimisrühma kompetentsi kuulub erinevate analüütiliste meetodite ning protseduuride väljatöötamine ning kasutamine mitmesuguste ühendite ja segude analüüsiks keerulistes maatriksites. Rühmas on spetsialistid lahutusmeetodite - eelkõige kapillaarelektroforeesi alal juhtivus- ja optilise detekteerimisega, aga ka vedelik ja gaasi kromatograafia alal. Samuti on kompetents ning vahendid erinevate ekstraktsioonimeetodite kasutamiseks.Rühmas on välja arendatud erinevaid modifitseeritud (poorseid) materjale – aerogeele, mida kasutatakse põhiliselt adsorbentidena ning katalüsaatoritena elektrokeemias ja veepuhastuses.Töötatakse erinevate analüütiliste meetodite (HPLC-MS, GC-MS, kapillaarelektroforeesil (KE) põhinevate) väljatöötamisega ja kasutamisega mitmesuguste ühendite ja segude (keemiarelvad, narkootikumid, bioaktiivsed ained, fütokemikaalid, toiduained, biomassi laguproduktid) analüüsiks keerulistes maatriksites nagu keskkonnaproovid, kehavedelikud ja taimeekstraktid.
The members of the group have competence indevelopment of analytical methods and procedures and use of these in analysis of differentcompounds and mixtures in complicated matrices. The group has good specialists in separationmethods: gas and liquid chromatography andespecially in capillary electrophoresis where theyuse a wide variety of detectors: electrical, opticaland mass spectrometrical. The group has alsocompetence and means for supercritical extraction for a wide range of extraction parameters.The group has provided recognised results ondevelopment of porous materials – aerogels andthese have been taken into use as adsorbents inanalytical separation and catalysts in electrochemistry and water purification.The aim of the present research is developmentand application of a variety of analytical methodologies (primarily capillary electrophoresis)for analysis of different classes of compounds(banned chemicals, drugs, polyphenols, fermentable sugars) in complex matrixes such asenvironmental samples, body fluids, biomass andherbal extracts. The results obtained will be usedto develop an expert system using quantitativestructure-activity relationship (QSAR).Miniaturization of capillary electrophoresis apparatus is an important feature, which providesan opportunity to perform on-site analyzes.
Võtmesõna
lahutusmeetodid
separation methods
miniaturiseerimine
miniaturization
narkootikumid
fütokemikaalid
phytochemicals
antioksüdatiivsus
antioxidativity
biomass
ioonsed vedelikud
ionic liquids
eutektilised segud
deep eutectic solvents
banned chemicals
modifitseeritud materjalid
Tähtsamad tulemused
Grupi olulisemateks tulemusteks on ravim- ja toidutaimedes ning biomassis leiduvate bioaktiivsete komponentide ekstraktsiooni, fraktsioneerimise ja analüüsimeetodite arendamine ning ekstraktide/fraktsioonide antioksüdatiivsete ja antibakteriaalsete (Borrelia-vastaste) omaduste testimine.2023. aastal uuriti kolme erineva Eestis levinud madara liigi (Galium verum, Galium aparine ja Galium mollugo) fütokeemilist koostist ning antioksüdantset aktiivsust. Uuriti ka erinevate Makaroneesia taimede (A. frutescensi, C. symphytifolius, H. gardnerianum, L. azorica, M. canariensis, S. canariensis ja W. aristata) vesi-etanool ekstraktide toimet Vahemere puuviljakärbse (C. capitata) vastu.
Most important part of the research was connected to the development and evaluation of an automatic portable capillary electrophoresis instrument, prototype II of Narcotester (DrugHunter). The CE system was coupled to UVC fluorescence detector providing 225–255 nm exitation wavelength range for the determination of the abuse of illegal drugs in oral fluid in situ. This portable Narcotester was employed for determination of natively fluorescensing drugs,such as cocaine, cocaethylene, seven amphetamines, fentanyl, LSD, tetrahydrocannabinol and cannabidiol. Based on this portable apparatus,several electrophoretic methodologies were developed, validated and successfully utilized for the determination of illegal abuse of drugs during music festivals in Estonia and Latvia.